Coding
Part:BBa_K4467003:Design
Designed by: Eloise Paine Group: iGEM22_UNSW_Australia (2022-10-10)
PstHXT1 (from Puccinia striiformis var. striiformis)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 878
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 715
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 573
Illegal BsaI.rc site found at 1494
Design Notes
Codon optimisation was performed for expression of this part in NEB TURBO E.coli and NEB T7 Express E. coli.
Source
PstHXT1 encodes for a sequence found in the wheat rust pathogen Puccinia striiformis f. sp. tritici (Pst) (Chang et. al., 2020).
References
Chang, Q. et al. (2020) "Hexose transporter Ps HXT1‐mediated sugar uptake is required for pathogenicity of wheat stripe rust", Plant Biotechnology Journal, 18(12), pp. 2367-2369. doi: 10.1111/pbi.13398.