Coding

Part:BBa_K4467003:Design

Designed by: Eloise Paine   Group: iGEM22_UNSW_Australia   (2022-10-10)


PstHXT1 (from Puccinia striiformis var. striiformis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 878
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 715
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 573
    Illegal BsaI.rc site found at 1494


Design Notes

Codon optimisation was performed for expression of this part in NEB TURBO E.coli and NEB T7 Express E. coli.

Source

PstHXT1 encodes for a sequence found in the wheat rust pathogen Puccinia striiformis f. sp. tritici (Pst) (Chang et. al., 2020).

References

Chang, Q. et al. (2020) "Hexose transporter Ps HXT1‐mediated sugar uptake is required for pathogenicity of wheat stripe rust", Plant Biotechnology Journal, 18(12), pp. 2367-2369. doi: 10.1111/pbi.13398.